Team:BCCS-Bristol/Calendar-Notebook/21 July 2008
From 2008.igem.org
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OD measurement
The OD of both strains was measured over a day so that we would know whether the bacteria were in their log phase in any experiments requiring innoculation of bacteria.
A, B and C show 100, 500 and 200µl innoculations respectively.
Time (minutes) | OD600 (1.5 diluted) |
| |||||
MG1655 | MC1000 | ||||||
A | B | C | A | B | C | ||
0 | 0.0016 | 0.0042 | 0.017 | 0.0016 | 0.0044 | 0.0174 | |
60 | 0.008 | 0.013 | 0.073 | 0.006 | 0.017 | 0.068 | |
80 | 0.011 | 0.052 | 0.109 | 0.009 | 0.046 | 0.123 | |
100 | 0.019 | 0.059 | 0.142 | 0.017 | 0.067 | 0.167 | |
120 | 0.029 | 0.076 | 0.169 | 0.024 | 0.083 | 0.183 | |
140 | 0.039 | 0.095 | 0.213 | 0.039 | 0.144 | 0.226 | |
160 | 0.063 | 0.136 | 0.26 | 0.053 | 0.147 | 0.275 | |
180 | 0.074 | 0.166 | 0.276 | 0.069 | 0.186 | 0.298 | |
200 | 0.105 | 0.207 | 0.301 | 0.089 | 0.22 | 0.316 | |
220 | 0.134 | 0.25 | 0.355 | 0.116 | 0.268 | 0.331 | |
240 | 0.178 | 0.276 | 0.386 | 0.151 | 0.293 | 0.341 | |
260 | 0.233 | 0.329 | 0.419 | 0.198 | 0.325 | 0.372 | |
280 | 0.239 | 0.334 | 0.435 | 0.203 | 0.326 | 0.375 | |
300 | 0.263 | 0.363 | 0.473 | 0.238 | 0.34 | 0.388 | |
320 | 0.296 | 0.391 | 0.491 | 0.27 | 0.364 | 0.412 | |
340 | 0.319 | 0.425 | 0.511 | 0.29 | 0.378 | 0.429 | |
360 | 0.32 | 0.435 | 0.512 | 0.294 | 0.382 | 0.432 | |
380 | 0.392 | 0.542 | 0.514 | 0.311 | 0.434 | 0.438 | |
400 | 0.435 | 0.549 | 0.588 | 0.348 | 0.457 | 0.497 | |
420 | 0.47 | 0.52 | 0.487 | 0.382 | 0.462 | 0.504 | |
440 | 0.483 | 0.586 | 0.633 | 0.477 | 0.437 | 0.516 | |
460 | 0.498 | 0.482 | 0.629 | 0.411 | 0.484 | 0.517 | |
480 | 0.574 | 0.643 | 0.721 | 0.451 | 0.516 | 0.524 | |
500 | 0.607 | 0.66 | 0.73 | 0.455 | 0.514 | 0.515 | |
520 | 0.65 | 0.673 | 0.723 | 0.453 | 0.522 | 0.523 |
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