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Team:University of Washington/Project
From 2008.igem.org
Bacterial Conjugation
The bacteria will then build the conjugation machinery, in response to the AHL produced from the yeast. This is being worked on in two separate ways on two different genes.
Two major genes in regulating IncP conjugation are KorA, a global regulation located on the RP4 plasmid, and TrbA, a gene that produces a transcriptional factor that controls the transcription of the TrbB operon, which contains the essential genes for building the conjugation pili and other parts of the conjugation tube.
Illustration of Tra1 Conjugation Machinery
Schröder, G., S. Krause, E. L. Zechner, B. Traxler, H. J. Yeo, R. Lurz, G. Waksman, and E. Lanka. 2002. TraG-like proteins of DNA transfer systems and of the Helicobacter pylori type IV secretion system: inner membrane gate for exported substrates? J. Bacteriol. 184:2767-2779. [http://www.ncbi.nlm.nih.gov/pubmed/11976307].
UW Lab Links
Protocols
BioBrick Parts Used
UW Glycerol Stocks
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