Virginia/25 July 2008

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Overnight Results

  • EVERYTHING WORKED! all ligations were successful thanks to the new T4 ligase
  • Also the E0840 plate from the day before now had colonies


Accomplished

  • Made small overnight broth of all assemblies
    • Broth of A30 (RFP + GFP) received 10ul of IPTG to induce pLac in I715039
  • Digested pSB1A10 and B1002 with EcoRI and PstI and ligated, transformed


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