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From 2008.igem.org

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Contents 1 Construction of pRL1383a 2 Methods 3 Results 4 Discussion

Construction of pRL1383a

• The BioBrick parts of pRL1383a are to be ligated in a series of experiments.

Methods

Round 1 of Restriction Digest and Ligation

Restriction Digest & Ligation: Round 1

Name	Digest 1	Digest 2	Ligation to	Product
mob	XbaI & PstI	n/a	[http://partsregistry.org/Part:BBa_B0024 BBa_B0024]	TT-mob
rep	XbaI & PstI	n/a	[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]	Plac-rep
oriV	XbaI & PstI	n/a	[http://partsregistry.org/Part:pSB1A3 pSB1A3]	pSB1A3::oriV
aadA region	XbaI & PstI	n/a	[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]	Plac-aadA
oriT	XbaI & PstI	n/a	[http://partsregistry.org/Part:pSB1A3 pSB1A3]	pSB1A3::oriT
pSB1A3	XbaI & PstI	n/a	see oriT and oriV	see oriT and oriV
[http://partsregistry.org/Part:BBa_B0024 BBa_B0024]	SpeI	PstI	see mob region above	see mob region above
[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]	SpeI	PstI	see rep and aadA region above	see rep and aadA region above

Round 2 of Restriction Digest and Ligation

Restriction Digest & Ligation: Round 2

Name	Digest 1	Digest 2	Ligation to	Product
TT-mob
Results Discussion What was learned and how to do future experiments differently. [http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

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