Team:Hawaii/Notebook/2008-07-23

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Things we did today

Wetlab work

Gel for Plasmid Prep from Yesterday

Margaret
File:Plasmid prep.pdf
Plasmid Prep of pSB1A3, pSB1A2, pSB3K3, and a verification of pSMC121.

Biobrick Extraction

Margaret
  • extracted: I14032, pSB1A7, I51020, J23012 and transformed into DB3.1 cells. Plated on amp100. **the problem is that I14032 is Kan resistant. I introduced 1mM IPTG to the 2 hour 37°C incubation, but we will not see the results of this because of my antibiotics mix-up
  • on 7/24 there were 2 colonies on the pSB1A7 plate and 4 colonies on the I51020 plate, these were restreaked
  • on 7/25 the re-streaked plates were placed in 4°C.

Plasmid Prep pRL1383a-M

Krystle
  • 200 ml prep of the new pRL1383 plasmid with biobrick multiple cloning site

Checked for transformants from yesterday/restreaked

Krystle
  • GFP + tt = 5 colonies
  • GFP fusion + tt = 15 colonies
  • nir + rbs = 1 colony

Discussion

Quote of the Day

[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]