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Team:Hawaii/Project
From 2008.igem.org
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Overall Project
We chose to focus on cyanobacteria because it efficiently uses light, water, carbon dioxide and trace minerals for its growth and accumulation of carbon based biomass. Cyanobacteria are frequently studied for their ability to harness the power of photosynthesis in order to produce a wide variety of useful products including bio-fuels and -polymers. Such tasks are accomplished by these ‘little green factories’ with a minimal input of salts, light, and carbon dioxide. We aim to expand the availability of BioBrick vectors to cyanobacteria in order to “open source” the current BioBrick registry to a greater range of organisms.
We plan to engineer:
- 1) a mobilizable broad-host-range BioBrick vector that can be used to transfer genetic information between E. coli and Synechocystis sp. 6803, with the future possibility of transforming plants via Agrobacterium and other bacteria transformable by RSF1010 based plasmids;
- 2) a cassette for protein export from Synechocystis; and
- 3) the nitrate-inducible cyanobacterial nir promoter.
The functionality of the parts we engineer will be demonstrated by achieving inducible protein production and export of GFP construct introduced into Synechocystis using our novel BioBrick mobilizable shuttle vector.
Project Details
- Part A: Mobilizable Broad-Host-Range Plasmid
- Part B: Cyanobacterial protein secretion system
Results
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
