Illinois/17 July 2008

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Antibody/GPCR Fusion

  • Consulting meeting with Huimin Zhao (Associate Professor, Chemical and Biomolecular Engineering)

LAB WORK

Receptor Tyrosine Kinase method

E.Coli Media

LB medium, per liter

10g tryptone

5g yeast extract

5g NaCl

1 mL 1N NaOH

15g (agar for plates)

1. antibody DNA resuspended in TE buffer, 0.1 μg/μL

2. 5mL LB inoculated with single colony DH5a pro

3. Incubated at 37 degrees overnight

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