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Wisconsin: Lignin Project/27 June 2008
From 2008.igem.org
Team Fungus:
Plate with 1:3 insert to vector ratio had 2 colonies
Plate with 0:1 vector had 1 colony
Rest had zero colonies
We streaked and plated the two colonies from the 1:3 onto two new LB + Kan plates
Made more LB + Kan plates
Team Sorbitol
Checked the PCR product from last night on a gel and found several bands to work again (700bp).
The product and pBAD30 were digested with Xba1 and HindIII restriction enzymes.
They were verified to work on a gel and extracted using a Qiagen Gel Extration Kit.
Unfortunately not enough DNA was obtained to perform a ligation.
