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Wisconsin: Lignin Project/2 July 2008
From 2008.igem.org
Team Sorbitol
The pBAD30 was harvested using a Qiagen Miniprep kit and digested as described before.
We were able to extract enough plasmid DNA to perform a ligation.
We then set up a ligation with the following rations of vector to insert: V:I 3 1 1 1 1 3 1 5 1 0 0 1
alowed to ligate at 4C overnight.
