Wisconsin: Lignin Project/11 July 2008

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Team Sorbitol

Froze down 3 400ul samples of the overnight DH5a-pBAD30-Srld E.coli for future use.

Harvested the DNA from the pBAD30, PBAD30-srlD, and pBAD18 using a miniprep kit.

created chemically competent stocks of MG1655, JW3890 and RL357.

The stocks were frozen down and kept at -80C for future use.