Team:Mississippi State/29 July 2008

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  1. Prepare low salt LB plates w/ 50ug/ml BLAST
  2. Prepare blast stock solution, 50mg + 1ml
  3. make new LB plates
  4. make YPD plates
  5. prepare ligation mix using T4 Dna Ligase and LiP and pPIC6
  6. spread x-33 yeast cells onto ypd plates, place in 28C to incubate