Team:Mississippi State/4 August 2008

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  1. Repeat digestion protocol using NotI, EcoRI, LiP, and pPIC6
  2. prepare and run 1% agarose gel, cut out products
  3. perform gel purification using QIAgen kit
  4. prepare and run 1% agarose gel
  5. grow x-33 yeast in YPD medium overnight
  6. make ligation mix with T4, Lip, and pPIC6
  7. grow overnight culture of xl1blue
  8. do transformation using ligation mix, spread onto plates, incubate overnight
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