Team:Hawaii/Meeting/2008-05-22

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Agenda

Updates
1. Annotation of vector with Vector NTI (Register and we can save our data)
Some advice
1. Working with the Registry:
  1. the wells (spots) have parts, there is a picture of it run on a gel, and there is a verification with antibiotic plates
  2. When we test parts out, use color coding on plates for different antibiotics
  3. Should we use 96 well plates for high throughput screening of parts?
2. Decide input and output of project
3. Make plasmid biobrick compatible (we should make every part a biobrick, this way we can mix and match parts on a plasmid as needed)**don’t get left with one huge part that does not function the way we want it to, then start from zero.
Presentations:
1. Norman: the big idea behind using BioBricks (MIT teaching the teach notes). Biobrick assembly, parts category (RBS, Regulatory, DNA, RNA, Protein Coding, Terminators, {skipped conjugation}) Meeting Slides (PDF)
2. Margaret: devices
3. Krystle: chassis & plasmids

Krystle
1. read up on RSF1010 make proposal for plasmid project (post ASAP)
Norman
1. Buy a KVM
2. Shopping
Margaret
1. finish the annotation and figure out the functions of the components
2. Begin proposal on the plasmid, what do we want to keep, we can delete stuff we don’t need using PCR, learn how to design primers that can delete and make biobricks
Everybody
1. Prepare proposal for part B (the idea you are championing)
2. Biobricks come on plasmids, so we need to get transformation down
3. Finalize shopping list, order by June 10th (can’t order between 6/15-6/30)

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]