Team:Hawaii/Notebook/2008-08-15

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Things we did today

Wetlab work

Reconstruction of BB-pRL1383a

EtBr stained 1% agarose gel ran at 72V for ... Thirty-five microliters of RE digested BB-pRL1383a and fifty microliters of RE digested J33207 were loaded.
Grace
  • PCR amplification of J33207
  • ExoSAP'd PCR product
  • RE digested of J33207 and BB-pRL1383a (w/ GFP) with EcoRI and PstI
  • Ran digests on a 1% agarose gel at 72V for 1.5 hours.
  • Ladder did not run well. Bands not resolving well.
  • Redid PCR and RE digests with XbaI and PstI

Made 20X X-gal stock solution (20 mg/ml)

Grace

Drylab Work

Editing team website

Grace

Discussion

  • DH5α does not carry lacIq; therefore, IPTG is not neccessary to induce the lac promoter.
  • IPTG is necessary for induction in DB3.1 cells.

Quote of the Day

I'm waiting for the terminator to come in... - Krystle


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