Team:Valencia/Notebook/Protocols

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Glycerol Stock

2 ml Eppendorf containing:

Sterile Glycerol (stock 50 %) 1ml
Half culture plate


SD Medium

Yeast Nitrogen Base 0.67%
Glucose 2%
Agar 2%
Casamino acids 0.1%
Tryptophan 20 mg/l
Adenine 40 mg/l


SP liquid medium

Yeast Nitrogen Base 0.67%
Potassium phosohate 0.1%
Ammonium sulfate 0.12%
Glucose 0.05%
Lactic acid 2%
Casamino acids 0.1%
Tryptophan 20 mg/l
Adenine 40 mg/l


YPKAc Medium

Yeast extract 1%
Peptone 2%
Potassium acetate 2%
Tryptophan 20 mg/l
Adenine 40 mg/l


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