Edinburgh/2 July 2008

From 2008.igem.org

Revision as of 11:07, 20 August 2008 by Andhi (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Edinburgh iGEM 2008

Week 3

Wednesday 2 July 08

Digests of M10 and M11 with EcoRI, to determine orientation, were not very clear (Gel 7). Probably simpler just to sequence them, since we will need to check that the ends are intact in any case. Ordered mutagenic primers to remove the EcoRI sites:

primer glgcm1f: tgttgaaaaacctgctaaccc
primer glgcm1r: aattcgataattttatcgttctc
primer glgcm2f: ctcattctgcaacattgattcc
primer glgcm2r: ttcacgcgaacgcgcgag