Edinburgh/19 June 2008

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Edinburgh iGEM 2008

 

Week 1

Thursday 19 June 08

  • Eluted DNA for BioBrick E0240 (GFP) from square 1001, well 4B and used this to transform half of tube J25; other half was transformed with 1.5μl of J33207 (Edinbrick1) as a control to test the competence of the cells. Plated 100 microlitres of each to LA+amb+BW. Incubate at 37°C overnight.
    • Plate 1: JM109 transformation with E0240 eluted DNA.
    • Plate 2: JM109 transformation with J33207 plasmid DNA (control).