Team:Hawaii/Notebook/2008-08-23

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Things we did today

Wetlab work

Verification of transformants (cont.)

Grace
  • Plasmid prep of nir+rbs, rbs+GFP
  • Overnight RE digest of plasmid preps, nir plasmid, GFP plasmid with XbaI and SpeI

3A assembly

Grace
  • Gel purified RE digests
  • Ligated with pSB1A2:
  • nir+rbs
  • plac+rbs
  • rbs+GFP
  • GFP+tt
  • GFPf+tt
  • Transformed DB3.1 cells with 5μl ligation reaction

BB-pRL1383a MCS re-replacement

Grace
  • Gel purified RE digests
  • Ligated J33207 with BB-pRL1383a
  • Transformed DH5&alpha cells with 5 μl ligation reaction

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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