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Team:Montreal/Notebook
From 2008.igem.org
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Lab Progress
May 22nd, 2008: - Prepared TOP10 Competent cells for eventual transformation. - Performed Mini-prep on Reporter+ Cells - Performed Digest and Gel on Reporter plasmid extract -- no DNA present, suggest follow-up maxi-prep
May 25th, 2008: - Diluted Reporter cells 1/1000 with 5ul Kan/mL culture for 16h incubation at 5:30pm. To be used for Maxiprep at 9:30am-1:30pm.
May 28th, 2008: - Ran gel on Elowitz reporter DNA cut with EcoR1; 2 bands - 0.7 kb and 2.0 kb, confirms identity of reporter DNA. - Seeded syn-I and J-40001 into amp/kan LB and kan LB.
May 29th, 2008: - Growth of J-brick in culture - No growth of I-brick on culture - Seeded J-brick for Midi-Prep in 40mL LB with ampicillin - Transformed TOP10 cells with both I brick and Reporter Plasmid
June 2nd, 2008: - Growth of I-brick on culture - Midi-prep of both I and J brick followed by gel - Gel indicates no presence of DNA, will be confirmed by spectrophotometric assay
June 3rd, 2008: - Seeding of 5mL cultures of both I and J brick - Identity of colonies on I brick plates is suspect, must ensure that eventual DNA gel confirms exact restriction digest
