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Team:Hawaii/Notebook/2008-08-27
From 2008.igem.org
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Construction of p+r
- Grace
- Ran RE digested products on gel
- nir had three bands. Band at ~730bp = ?
- BB-pRL1383a -- still couldn't see cut out of GFP. Assumed double digest successful, too little DNA to see GFP.
- J33207 wrong size
- Extracted nir, C0012 vector, BB-pRL1383a from gel
- 3A assembly (ligation) of C0012 vector (pSB1A2) with:
- nir and rbs (B0034)
- plac and rbs(B0034)
- pSB1A2 (negative control)
- Transformed DB3.1 cells using 5 μl ligation reaction
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
