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Edinburgh/Notebook/Minipreps
From 2008.igem.org
M1-M6: pSB1A2+dxs transformants (plate 11). M2, M3, M4 and M6 all looked correct on gel 2. (30.06.08) - M2 was sequenced to confirm identity and was maxiprepped as X2.
M7: BABEL1+glgC transformant (plate 12). Did not look right on gel. (30.06.08)
M8-M12: BABEL2+glgC transformants (plate 13). M10 and M11 both looked good on a gel, but sequencing showed that both had the insert in the reverse orientation. (30.06.08)
M13-M18: pSB1A2+appY transformants. Gel 10 showed that M15 to M18, and possibly M14, all seemed to have inserts the right size. (09.07.08)
M19-M24: BABEL2+glgC clones after mutation of EcoRI site 2. EcoRI digests on *Gel 11* suggest that *M19, M21 and M22* may have lost the EcoRI site. (10.07.08)
M25-M30: possible rbs+dxs transformants from plate 34. (13.07.08)
M31-M36: pSB1A2+crtB clones from plate 35. (14.07.08)
M37-M42: pSB1A2+crtI clones from plate 36. (14.07.08)
M43-M47: possible BABEL2+glgC double mutants (both EcoRI sites gone). (19.07.08)
M48: a possible pSB1A2+rbs+dxs clone. (19.07.08)
M49-M54: pSB1A2-rbs+crtI. (29.07.08: CF)
M55-M60: Putative pSB1A2+pZntA (L23). (29.07.08: Yan, Nimisha) - M50 sequenced (31.07.08: CF)
M61-M66: Putative pSB1A2+rbs+crtE (L22). (29.07.08: Yan, Nimisha) - M63 sequenced (31.07.08: CF)
M67: rbs+crtB (L21) (29.07.08: Yan, Nimisha) - Sequenced (31.07.08: CF)
M68-M71: pSB1A2+glgC-mut1,2 from plate 73 (31.07.08: AM, Yan)
M72-M75: pSB1A2+rbs+dxs from plate 74 (31.07.08: AM, Yan)
M76-M79: BABEL2+crtE from plate 74 (31.07.08: AM, Yan)
M80-M83: pSB1A2+rbs+appY from plate 83. (04.08.08: Yan, OG)
M84-M85: pSB1A2+rbs+crtB from plate 84. (04.08.08: Yan, OG)
M86-M90: pSB1A2+cenA from plate 85. (04.08.08: Yan, OG)
M91-M96: pSB1A2+cex from plate 86. (04.08.08: Yan, OG)
M97-M104: pSB1A2+cenA from plate 85. (05.08.08: Yan)
M105-M108: pSB1A2+cex from plate 85. (05.08.08: Yan)
M109-M112: pSB1A2+crtB+crtI and plate 102 (11.08.08: OG)
M113-M116: pSB1A2+glgC-mut1,2 and plate 103 (11.08.08: OG)
M117-M120: pSB1A2+glgC-mut1,2,3 and plate 94 (11.08.08: OG)
M121 to M123: pSB1A2-crtY (12.08.08: OG)
M124 to M126: pSBA12-rbs+crtY (12.08.08: OG)
M127 to M130: pSB1A2-PcstA from plate 115 (13.08.08: OG)
M131 to M134: Minipreps of dxs+crtE (1,2,3,4) Note: M132 might be M136 (13.08.08: OG)
M135 to M138: Minipreps of dxs+lims (1,2,3,4) Note: M136 might be M132 (13.08.08: OG)
M139 to M143: Minipreps of pSB1A2 +CrtE (1 to 4) Note: M142 and M143 both made from culture 4.(15.08.08: YAN/OG)
M144 to M147: pSB1A2+cenA from plate 129 - Methanol used in preparation rather than ethanol! (18.08.08: OG)
M148 to M151: pSB1A2+cex from plate 129. (18.08.08: OG)
M152 to M155: pSB1A2+dxs+crtE from plate 130 (18.08.08: OG)
