Team:Chiba/jk/β
From 2008.igem.org
β班の実験ノート
Contents |
Week 1
- 17 August, 2008
- Transformation
- BBa_R0040 [http://partsregistry.org/Part:BBa_R0040](2008) -> No colonies on plates
- BBa_C0070 [http://partsregistry.org/Part:BBa_C0070](2008) -> No colonies on plates
- BBa_C0076 [http://partsregistry.org/Part:BBa_C0076](2008) -> No colonies on plates
- BBa_C0077 [http://partsregistry.org/Part:BBa_C0077](2008) -> No colonies on plates
- BBa_C0078 [http://partsregistry.org/Part:BBa_C0078](2008) -> No colonies on plates
- BBa_C0079 [http://partsregistry.org/Part:BBa_C0079](2008) -> No colonies on plates
- 18 August, 2008
- Transformation
- BBa_C0070 [http://partsregistry.org/Part:BBa_C0070](2007)
- BBa_C0070 [http://partsregistry.org/Part:BBa_C0070](2006)
- BBa_C0076 [http://partsregistry.org/Part:BBa_C0076](2007)
- BBa_C0076 [http://partsregistry.org/Part:BBa_C0076](2006) -> No colonies on plates
- BBa_C0078 [http://partsregistry.org/Part:BBa_C0078](2007)
- BBa_C0078 [http://partsregistry.org/Part:BBa_C0078](2006)
- BBa_C0170 [http://partsregistry.org/Part:BBa_C0170](2007)
- BBa_C0170 [http://partsregistry.org/Part:BBa_C0170](2006)
- BBa_C0178 [http://partsregistry.org/Part:BBa_C0178](2007)
- BBa_C0178 [http://partsregistry.org/Part:BBa_C0178](2006)
- --->(20/8)Mini prep
- 19 August, 2008
- PCR
- BBa_I9026 [http://partsregistry.org/Part:BBa_I9026](2007)
- BBa_I9026 [http://partsregistry.org/Part:BBa_I9026](2006)
- BBa_I9030 [http://partsregistry.org/Part:BBa_I9030](2007)
- BBa_I9030 [http://partsregistry.org/Part:BBa_I9030](2006)
- [http://partsregistry.org/Part:BBa_S3154| BBa_S3154](2007)
- [http://partsregistry.org/Part:BBa_S3154| BBa_S3154](2006)
- [http://partsregistry.org/Part:BBa_F2620| BBa_F2620](2007)・・・control
- --->Gel Check
- [http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2007)
- [http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2006)
- [http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2007)
- [http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2006)
- [http://partsregistry.org/Part:BBa_S3154| BBa_S3154](2007)
- [http://partsregistry.org/Part:BBa_S3154| BBa_S3154](2006)
- [http://partsregistry.org/Part:BBa_F2620| BBa_F2620](2007)・・・control
- 20 August, 2008
- (18/8)--->Mini prep
- [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007)
- [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006)
- [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2007)
- [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007)
- [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006)
- [http://partsregistry.org/Part:BBa_C0170| BBa_C0170](2007)
- [http://partsregistry.org/Part:BBa_C0170| BBa_C0170](2006)
- [http://partsregistry.org/Part:BBa_C0178| BBa_C0178](2007)
- [http://partsregistry.org/Part:BBa_C0178| BBa_C0178](2006)
- --->Gel Check
- [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007) -> OK
- [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006) -> OK
- [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2007) -> None
- [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007) -> None
- [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006) -> OK
- [http://partsregistry.org/Part:BBa_C0170| BBa_C0170](2007) -> OK
- [http://partsregistry.org/Part:BBa_C0170| BBa_C0170](2006) -> OK
- [http://partsregistry.org/Part:BBa_C0178| BBa_C0178](2007) -> OK
- [http://partsregistry.org/Part:BBa_C0178| BBa_C0178](2006) -> OK
- --->Digestion
- [http://partsregistry.org/Part:BBa_C0170| BBa_C0170](2007)
- [http://partsregistry.org/Part:BBa_C0170| BBa_C0170](2006)
- [http://partsregistry.org/Part:BBa_C0178| BBa_C0178](2007)
- [http://partsregistry.org/Part:BBa_C0178| BBa_C0178](2006)
- 21 August, 2008
- (20/8)--->PCR
- [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007)
- [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006)
- [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2007)
- [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007)
- [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006)
- --->Gel Check
- [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007) -> OK
- [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006) -> OK
- [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2007) -> None
- [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007) -> None
- [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006) -> None
- Transformation
- [http://partsregistry.org/Part:BBa_S03154| BBa_S03154](2007)
- [http://partsregistry.org/Part:BBa_S03154| BBa_S03154](2006)
- [http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2007)
- [http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2006)
- [http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2007)
- [http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2006)
- 22 August, 2008
- Transformation
- [http://partsregistry.org/Part:BBa_J13002| BBa_J13002](2007)
- [http://partsregistry.org/Part:BBa_J13002| BBa_J13002](2006)
- [http://partsregistry.org/Part:BBa_J04500| BBa_J04500](2007)
- [http://partsregistry.org/Part:BBa_J04500| BBa_J04500](2006)
- (Locate & 2 Punch ~iyama ver.~)
- [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2008) -> No colonies on plates
- [http://partsregistry.org/Part:BBa_C0077| BBa_C0077](2008) -> No colonies on plates
- [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2008) -> No colonies on plates
- 23 August, 2008
- --->Mini prep
- [http://partsregistry.org/Part:BBa_S03154| BBa_S03154](2007)
- [http://partsregistry.org/Part:BBa_S03154| BBa_S03154](2006)
- [http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2007)
- [http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2006)
- [http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2007)
- [http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2006)
- --->Digestion
- [http://partsregistry.org/Part:BBa_S03154| BBa_S03154](2007)
- [http://partsregistry.org/Part:BBa_S03154| BBa_S03154](2006)
- [http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2007)
- [http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2006)
- [http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2007)
- [http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2006)
- [http://partsregistry.org/Part:BBa_J04500| BBa_J04500](2007)
- --->Gel Check
- [http://partsregistry.org/Part:BBa_S03154| BBa_S03154](2007)
- [http://partsregistry.org/Part:BBa_S03154| BBa_S03154](2006)
- [http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2007)
- [http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2006)
- [http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2007)
- [http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2006)
Week 2
- 24 August, 2008
- (22/8)--->Mini prep
- [http://partsregistry.org/Part:BBa_J13002| BBa_J13002](2007)
- [http://partsregistry.org/Part:BBa_J13002| BBa_J13002](2006)
- [http://partsregistry.org/Part:BBa_J04500| BBa_J04500](2007)
- [http://partsregistry.org/Part:BBa_J04500| BBa_J04500](2006)
- --->Digestion
- [http://partsregistry.org/Part:BBa_J13002| BBa_J13002](2007)
- [http://partsregistry.org/Part:BBa_J13002| BBa_J13002](2006)
- [http://partsregistry.org/Part:BBa_J04500| BBa_J04500](2007)
- [http://partsregistry.org/Part:BBa_J04500| BBa_J04500](2006)
- --->Gel Check
- [http://partsregistry.org/Part:BBa_J13002| BBa_J13002](2007)
- [http://partsregistry.org/Part:BBa_J13002| BBa_J13002](2006)
- [http://partsregistry.org/Part:BBa_J04500| BBa_J04500](2007)
- [http://partsregistry.org/Part:BBa_J04500| BBa_J04500](2006)
- 25 August, 2008
- (24/8)--->Digestion
- [http://partsregistry.org/Part:BBa_J04500| BBa_J04500](2007)
- [http://partsregistry.org/Part:BBa_R0010| BBa_R0010](2007)
- --->Gel Check
- [http://partsregistry.org/Part:BBa_J04500| BBa_J04500](2007)
- [http://partsregistry.org/Part:BBa_R0010| BBa_R0010](2007)
- 26 August, 2008
- 27 August, 2008
- PCR
- Colony PCR of 5 colonies from ligation plates (27/8) and one from control plate([http://partsregistry.org/Part:BBa_F2620| BBa_F2620](2007)).
- 30 August, 2008
Week 3
- 31 August, 2008
- 1 September, 2008
Week 4
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