Team:Hawaii/Notebook/2008-08-29

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Things we did today

Wetlab work

PCR

Grace
  • nir and J33207 from plasmid preps
  • Verified products on 1.2% agarose gel

Plasmid preps

Grace
  • Resuspended plasmid preps in TE buffer
  • Verified preps on 1.2% agarose gel

Restriction digest

Grace
  • BB-pRL1383a and J33207 with XbaI and PstI
  • nir with EcoRI and SpeI
  • C0012 with EcoRI and PstI

Sequencing continued from 8-27

Margaret


  • Exo-Sap:OriV clones 1 thru 4, aadA(from BB registry) clones 5&9, aada(from pRL1383a) clones 3&6
  • OriV clones 1 thru 4, aadA(from BB registry) clones 5&9, aada(from pRL1383a) clones 3&6.
  • The omega interposon DNA was not submitted because there were multiple bands-->tomorrow I will work some more on this
  • also submitted some of Grace's constructs

Colony PCR

Margaret

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]


construct quantity(ng/ul)
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