Team:Hawaii/Notebook/2008-09- 4

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Revision as of 01:15, 4 September 2008 by Gracek (Talk | contribs)
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Things we did today

Wetlab work

Re-replacement of BB-pRL1383a MCS

Grace
  • Treated inactivated RE digest reaction with SAP
  • Checked DNA concentration of BB-pRL1383a (---ng/μl)
  • Ligated J33207 with BB-pRL1383a
  • Negative controls = BB-pRL1383a ligated to self, J33207 ligated to self
  • Transformed into DH5α cells

3A construction of plac+rbs

Grace
  • Ligated:
  • C0012V (SAP treated) + plac + rbs (B0034 back ligation)
  • C0012V + plac + rbs (B0034 back ligation)
  • Negative controls = C0012V (SAP treated and no SAP treatment) ligated to self, plac + rbs ligated w/o vector
  • Transformed into DB3.1 cells

Drylab Work

Name of Task

name of person/people who performed the task
  • Summary of task and what was done. Link to experiment for detailed notes if necessary.
  • e.g. read through papers, worked on proposal, etc.


Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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