Rensselaer/25 August 2008

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Promoter Group

Restriction Digest of Ligation Colonies Expressing RFP

H2O 31ul 31ul Buffer 4 4ul 4ul DNA: Ligation DNA Sample 19 2ul " " Sample 20 2ul Enzymes: MscI (3U/ul) 2ul 2ul EcoRI (20U/ul) 1ul 1ul Total Volume 40ul 40ul

Incubate @ 37C ON