Team:Hawaii/Notebook/2008-09- 5

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Things we did today

Wetlab work

Construction of Broad-Host-Range Vector Intermediates

Margaret
  • Plasmid Prep: Everything that didn't work yesterday
  • 2 Plac + B0030, 1 Plac + B0034, aadA(BB)colony 5, pSB3k3, pSB1A3
  • Restriction Digest: same as Yesterday
  • Ligation
  • Transformation into DH5alpha (we need more competent cells)

Drylab Work

Sequencing

Margaret
  • Analysis of Sequencing submitted (oriV1-4, 2 aadA(BB), 2 aadA (pRL))


Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]