Team:Hawaii/Notebook/2008-09-11

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Things we did today

Wetlab work

Construction of p+r + s(+gf)

Grace
EtBr stained 4% agarose gel ran at 47V for 2.5 hours. Thirty microliters of RE digest reaction were loaded into each well.
  • Ran RE digests from last night on gel
  • Extracted DNA from gel
  • slr1, pilA, nir+rbs
  • (From yesterday) slr1+GFPf, pilA+GFPf (8/12 and 8/13 transformations)
  • Ligated:
  • pSB1A3 and nir+rbs with slr1, pilA, slr1+GFPf, pilA+GFPf (8/12 and 8/13 transformations)
  • nir+rbs (on SpeI/PstI digested pSB1A2 plasmid) with slr1, pilA, slr1+GFPf, pilA+GFPf (8/12 and 8/13 transformations)
  • plac+rbs (on SpeI/PstI digested pSB1A2 plasmid) with slr1, pilA, slr1+GFPf, pilA+GFPf (8/12 and 8/13 transformations)
  • Transformed into DH5α (3A ligations) or DB3.1 (2-way ligations) cells

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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