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Things we did today

Wetlab work

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C0012V (sequence to see what it was): EcoRI/XbaI/SpeI/PstI sites present. Insert aligns to sequence for lacI (C0012 insert, vector was uncut)
C0012V-SAP'd (sequence to see what it was): EcoRI/XbaI/SpeI/PstI sites present. Insert aligns to sequence for lacI (C0012 insert, vector was uncut)
slr1+GFPf

3A:nrp: EcoRI/PstI sites present. 82bp insert aligns to sequence for E. coli flagellar biosynthesis RNA polymerase sigma factor
3A:nrpg12-2: EcoRI/PstI sites present. 759bp insert aligns to sequence for E. coli fumarate reductase (anaerobic) catalytic and NAD/flavoprotein subunit
3A:nrpg12-6: EcoRI/PstI sites present. 899bp insert aligns to sequence for E. coli protein C5 component of RNAse P
3A:nrs2: EcoRI/PstI sites present. 85bp insert aligns to sequence for E. coli diaminopimelate decarboxylase, PLP-binding
3A:nrs3: SpeI/PstI sites present. Insert aligns to pilA sequence and pSB1A3 transcriptional termination stem loop
3A:nrsg10: SpeI/PstI sites present. Insert aligns to pilA + GFPf
pilA+GFPf12: GFPf only
plac+rbs1 (sequence to see what it was): EcoRI/XbaI/SpeI/PstI sites present. rbs (B0034) only
plac+rbs2 (sequence to see what it was): EcoRI/XbaI/SpeI sites present. Insert showed 97% similarity to RP4 origin of replication
plac+rbs12 (sequence to see what it was): EcoRI/XbaI/SpeI/PstI sites present. Insert is GFP only.

Please throw away old plates in 4C fridge. Some have fungi growing on them already.

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

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