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Team:Freiburg/Project
From 2008.igem.org
Overall Project:
This year´s main project is the attempt to create an "artificial receptor-system", featuring extra- and intracellular modules as well as suitable transmembrane regions.
The intracellular domaine of our receptor is build by halfes of split reporter-proteins that can be reassembled to produce readable output. Each one of these molecule-halfes
is connected to it´s extracellular domaine by a single-span transmembrane-helix. The extracellular or detecting domaine consists of a protein or peptide with the ability to bind the presented stimulus.
Now, if a system with two matching receptors is presented the stimulus in a strict, pairwise spacial arrangement, the receptor-molecules are brought together,
the split reporter-protein reassembles inside the cell and the output can be detected.
We employ so-called "Origami-DNA" to create the exactly defined stimulus-patterns that are needed to activate our receptors.
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