Revision as of 23:26, 21 September 2008 by MargaretRuzicka (Talk | contribs)

Birds Eye View Team	Projects	Events	Resources
Sponsors	Experiments	Milestones	Protocols
	Notebook (t)	Meetings (t)

Things we did today

Wetlab work

Margaret

Included in my last transformation were pSB1A3, de-phosphorylated pSB1A3, and restriction digested (E,P) pSB1A3. All had colonies on DH5 alpha, and I want to see what they are.
Colony PCR, 62°C annealing, 1 minute extension.
Run 2%gel to compare them to colonies of curious size. I will also run the 8 rep colonies from this transformation that I ran a PCR on yesterday.
PCR order: 2 pSB1A3, restriction digested (E,P) pSB1A3, & 2 de-phosphorylated pSB1A3, (+) pSB1A3 from a plasmid prep, and (-) water. All using VF2&VR primers.

name of person/people who performed the task

Summary of task and what was done. Link to experiment for detailed notes if necessary.
e.g. read through papers, worked on proposal, etc.

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]