"
Team:Hawaii/Notebook/2008-09-21
From 2008.igem.org
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Transformation from last week
- Margaret
- Included in my last transformation were pSB1A3, de-phosphorylated pSB1A3, and restriction digested (E,P) pSB1A3. All had colonies on DH5 alpha, and I want to see what they are.
- Colony PCR, 62°C annealing, 1 minute extension.
- Run 2%gel to compare them to colonies of curious size. I will also run the 8 rep colonies from this transformation that I ran a PCR on yesterday.
- PCR order: 2 pSB1A3, restriction digested (E,P) pSB1A3, & 2 de-phosphorylated pSB1A3, (+) pSB1A3 from a plasmid prep, and (-) water. All using VF2&VR primers.
Transformation of ligations into DH5α
- Grace
- Used 5μl of ligation reaction from yesterday
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
