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Team:NTU-Singapore/Notebook/2 June 2008
From 2008.igem.org
Monday 2nd June
- Hung(Greenbear), Zhen Fu
- Grow BW and W3110 E coli with CoE7 plasmid on agar plates:
- 1 LBA plate, half for BW, half for W3110 (should have no bacteria growth)
- 1 LB plate, half for BW, half for W3110
- Amplification of LacI-GFP ([http://partsregistry.org/wiki/index.php?title=Part:BBa_J04430 BBa_J04430]) and Terminator ([http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]) parts.
- Overnight incubation at 37 degrees C of these 3 agar plates:
- LB AmpR spread with 250ul cells with LacI-GFP plasmid.
- LB AmpR spread with 125ul cells with Terminator plasmid.
- LB KanR spread with 125ul cells with Terminator plasmid.
- Inoculation of bacteria containing Colicin E7 plasmid (without AmpR) received from Taiwan. We inoculated the cells in 4 cryotubes, which were then put into 37 degrees incubator for overnight incubation:
- 2 tubes each containing 5ml LB broth with Ampicilin. (which we expect not to see any bacteria in the next morning)
- 2 tubes each containing 5ml LB broth without Ampicilin.
- Grow BW and W3110 E coli with CoE7 plasmid on agar plates:
