Team:Montreal/Notebook

From 2008.igem.org

Revision as of 14:10, 9 June 2008 by Sayehd (Talk | contribs)
Small mcgill igem.jpg
Home The Team The Project Parts Submitted to the Registry Modeling Notebook

Lab Progress

May 21st, 2008: - Resctriction enzyme digest was done on the J-brick with EcoRI. Gel was run on the J-brick after the restriction digest. No DNA was detected on the gel (the ladder was visible on the gel).

May 22nd, 2008: - Prepared TOP10 Competent cells for eventual transformation. - Performed Mini-prep on Reporter+ Cells - Performed Digest and Gel on Reporter plasmid extract -- no DNA present, suggest follow-up maxi-prep

May 23rd, 2008: - Transformed Top10 cells with Puc19 to ensure that the competent cell procedure was successful. Growth was observed, therefore procedure was successful.

May 25th, 2008: - Diluted Reporter cells 1/1000 with 5ul Kan/mL culture for 16h incubation at 5:30pm. To be used for Maxiprep at 9:30am-1:30pm.

May 28th, 2008: - Ran gel on Elowitz reporter DNA cut with EcoR1; 2 bands - 0.7 kb and 2.0 kb, confirms identity of reporter DNA. - Seeded syn-I and J-40001 into amp/kan LB and kan LB.

May 29th, 2008: - Growth of J-brick in culture - No growth of I-brick on culture - Seeded J-brick for Midi-Prep in 40mL LB with ampicillin - Transformed TOP10 cells with both I brick and Reporter Plasmid

June 2nd, 2008: - Growth of I-brick on culture - Midi-prep of both I and J brick followed by gel - Gel indicates no presence of DNA, will be confirmed by spectrophotometric assay

June 3rd, 2008: - Seeding of 5mL cultures of both I and J brick - Identity of colonies on I brick plates is suspect, must ensure that eventual DNA gel confirms exact restriction digest

June 4th, 2008: -Midiprep was done on the I and the J-brick. Once the isopropanol was added, the J-brick midiprep looked clear (no DNA was eluted). DNA gel needs to be done to confirm presence of DNA in both cases.