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Judging/Variance/LCG-UNAM Mexico
From 2008.igem.org
Dear iGEM judges, > > The team LCG-UNAM-MEXICO is participating this year for the first > time, and I'm writing on behalf of the team to request permission > for submitting our Biobricks in non-standard vectors. > > Our project requires very tight regulation of E. coli nickel > extrusion pump (RcnA) through several regulatory upstream proteins. > Our Analysis suggest that we need to control very accurately the > production of several molecules and a crucial part of this requires > stable low copy-number vectors. For this reason we would like to use > pRK415 and pBBIMCS-5 derivatives as our plasmid backbones. We will > be adding Biobrick prefixx and suffix to both of them. We will also > make sure that our parts are easily extracted from the plasmid > either through digestion with commercially available enzymes or > through PCR. > > All this information will be shared to ensure that our Biobricks are > usefull for the community. > > Thanks for your time and consideration > > Sur Herrera Paredes > LCG-UNAM-MEXICO > Licenciatura en Ciencias Genómicas > Universidad Nacional Autónoma de México
