Alberta NINT/9 June 2008

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Revision as of 22:25, 9 June 2008 by Sdeane (Talk | contribs)

lab work (SD): 

            K102008 and K102008 negative control plates again had >100 colonies each.  
            Verified sequencing for K102002.
            Digested K102002 with BamHI, with NcoI, and with BamHI+NcoI to test the digestion.
            Ran DNA from digest on 2% agarose gel.
            Gel reveals that digest of K102002 with BamHI formed two fragments.  Conclusion: K102002 contains
            two BamHI restriction sites.  A closer examination of the sequences indicates BBa_E0433 (LacZ alpha)
            contains a BamHI restriction site.  A new version of LacZ alpha is required.
            


            BBa_I732018 is LacZ alpha but lacks the BamHI restriction site.  The DNA for this part is isolated 
            from the iGEM BioBricks parts registry book according to the standard procedure.
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