1. Separate transformant colonies (tranformation from previous day) inoculated to liquid LB with kanamycin.

Antoni:

1. Preparation of chemocompetent bacteria E. coli TOP10.

Cloning of protein A DNA to pET15b+Omp-alfa plasmid
Antoni

1. Digest pET15b+OmpA_alpha and pGeneart+A with NdeI and NotI, pET15b+OmpA_alpha dephosphorylation with CIAP
2. Gel electrophoresis of digested plasmids and gel-out of proper bands
3. Ligation of pET15b+alfa and A
4. Transformation of E. coli TOP10.
5. Transformants plating on LB + ampitilin.