Team:Warsaw/Calendar-Main/24 May 2008

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Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7

Michał K.:

  1. Isolation of hypothetical pMPM-T5 with transcription fusion from other colonies.
  2. Isolation of hypothetical pMPM-T5 with translation fusion from other colonies.
  3. Control digest of pMPM-T5 with transcription fusion with EcoRI and XhoI (2x Tango buffer).
  4. Control digest of pMPM-T5 with translation fusion with EcoRI and XbaI (2x Tango buffer).
  5. Gel electrophoresis (no proper colonies found).

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