Team:Illinois/Antibody GPCR Fusion Notebook

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!-- == 22nd July ==

  • Yeast obtained from Dr. Zhao


!-- == 24th July ==

  • Prepared liquid culture for DNA extraction
  • Made 1M Tris. Cl pH 8.0
  • Made 4M ammonium acetate


!-- == 22nd August ==

  • Attempted DNA extraction
    • Result: Failed
  • Obtained more yeast from Dr. Zhao


!-- == 25th August ==

  • Prepared overnight culture for DNA extraction (3:27pm)


!-- == 26th August ==

  • Attempted DNA extraction
  • Prepped overnight culture


!-- == 27th August ==

  • Performed PCR (FILL IN PCR TABLE)
  • Prepped 3 overnight cultures


!-- == 28th August ==

  • Extracted DNA from 4 cultures
  • Ran gel of PCR products (1.5%, 200V)
    • Result: No bands present



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