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Team:Illinois/Antibody GPCR Fusion Notebook
From 2008.igem.org
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!-- == 22nd July ==
- Yeast obtained from Dr. Zhao
!-- == 24th July ==
- Prepared liquid culture for DNA extraction
- Made 1M Tris. Cl pH 8.0
- Made 4M ammonium acetate
!-- == 22nd August ==
- Attempted DNA extraction
- Result: Failed
- Obtained more yeast from Dr. Zhao
!-- == 25th August ==
- Prepared overnight culture for DNA extraction (3:27pm)
!-- == 26th August ==
- Attempted DNA extraction
- Prepped overnight culture
!-- == 27th August ==
- Performed PCR (FILL IN PCR TABLE)
- Prepped 3 overnight cultures
!-- == 28th August ==
- Extracted DNA from 4 cultures
- Ran gel of PCR products (1.5%, 200V)
- Result: No bands present
!-- == 2nd September ==
- FILL IN PCR TABLE
!-- == 3rd September ==
- Ran gel
- Ladder lane 7
- Sample 7 spilled
- 1% agarose
- Too high
- 120V
- Too low
- 50 minutes
!-- == 8th September ==
- FILL IN PCR TABLE
- Prepped 4 overnight cultures
- Yeast dried out again
!-- == 9th September ==
- Signs of life in 3 of the cultures
- Wait until tomorrow
- Ran gel on PCR from 8th September
- 150V, 50 minutes
- No sign of DNA
- Ladder from Courtney
!-- == 10th September ==
- Ran gel again
- Split culture
- 150V, 50 minutes
- 0.75% gel
- Ladder from Courtney
!-- == 11th September ==
- FILL IN PCR TABLE
!-- == 12th September ==
- Isolated DNA from 8 cultures
- Ran gel
- 1% agarose
- 150V
- 38 mins
- Poor results
!-- == 15th September ==
- PCR PGK Promotor
- Finnzymes Phusion High Fidelity DNA Polymerase
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