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Team:Warsaw/Calendar-Main/9 May 2008
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Attribution analyzer
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Interactive list of topics
Preparation of pMPMT5+AID construct
Michał K.
Restriction
digest
of
pTrc99A-AID
with HindIII and NcoI (1x Tango buffer).
DNA electrophoresis and
isolation of DNA
encoding AID from proper band (600 bp).
1-DNA ladder; 2-pTRC99a-AID digested with NcoI and HindIII
Restriction
digest
of
pMPM-T5
with HindIII and NcoI (1x Tango buffer),
dephosphorylation
(CIAP).
DNA electrophoresis and
isolation
of
pMPM-T5
vector from proper band (4250 bp).
1-DNA ladder; 2-pMPM-T5 digested with NcoI and HindIII
Electrophoresis to estimate the concentration of isolated DNA.
Ligation
of isolated DNA fragments.
"
1-DNA ladder; 2-pTRC99a-AID digested with NcoI and HindIII
1-DNA ladder; 2-pMPM-T5 digested with NcoI and HindIII
