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Team:Valencia/prueba3
From 2008.igem.org
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Super titulo
Hola iGEMITOS
Probando
| Home | The Team | The Project | Parts Submitted to the Registry | Modeling | Notebook |
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| Home | The Team | The Project | Parts Submitted to the Registry | Modeling | Notebook |
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Introduction to Synthetic Biology 2008
Hot (and sexy) Yeast
Have you ever forgotten to keep your culture on the stove? Isn´t it annoying to lose several week's work? Tired of apologizing to your boss? WE HAVE THE SOLUTION!!! Valencia iGEM '08 Team heat it up in your lab!!!
Besetes en el culete!
--- No asustarse, estamos probando colores ---
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Main Project - [http://parts.mit.edu/igem07/index.php/Imperial/Infector_Detector/Introduction Infector Detector]Infector Detector is a system that will detect the presence of biofilm infections on urinary catheters by reporting on the presence of AHL, a signalling molecule used by E.coli, and output a fluorescent protein as a result. ([http://parts.mit.edu/igem07/index.php/Imperial/Infector_Detector/Introduction more...]) |
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Main Achievements
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- Discount DNA Synthesis from Geneart. iGEM's DNA synthesis partner offers iGEM teams highly discounted DNA synthesis: $0.20 per base and $35 shipping. Check out the resources box for more information.
- Are you having trouble transforming your DNA from the 2008 Spring DNA Distribution? Please stay tuned. We have sent out a hard copy of the first volume of out iGEM Newsletter which has information discussing this with tips on how to get better results. Download the online pdf version of the newsletter here.
Jaume quiere saber si se desmonta
Meri
Joaquina
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| Specification | Design Solution | System Level |
| Health & Safety - System must not contain living cells, or cause any harm to patient. | Use a Cell Free System e.g. Promega's S30 Cell Extract | Chassis |
| Shelf-life - System must have a shelf life of 7 days. | Use a Cell Free System that can be packaged and stored in a -80°C freezer for 7 days. | Chassis |
| Packaging and Application - System must be applied as a cream or spray. | Package the system in liquid form. | Chassis |
| Inputs - System must be sensitive to AHL concentration between 5-50nM. | Sensitive AHL receiver to detect low AHL levels - Part no. F2620. | Construct |
| Outputs - System must give a visual signal if AHL is present. | Couple AHL to a reporter system expressing fluoresent protein eg. RFP | Construct |
| Operating Conditions - System must operate within temperature 20°-30°C. | Gene expression systems and protein must be thermostable. Characterise a Cell Free System at these temperatures. | Construct & Chassis |
| Response Time - System needs to have a response time under 3 hours. | To be Determined - this is hard to design for. Protease Inhibitor of Cell Extract should ensure degradation of Visual Reporter is minimal. | Construct & Chassis |
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Links
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Brown iGem on Openwetware Brown iGem Official Website Check out our Videos! |
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News Updates
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