Cloning alpha-A and omega-A fusions on pKS

Michał L., Ewa, Marcin

1. Isolation of plasmids from transformants.
2. Control digest of plasmids with SacI+NotI (BamHI buffer).
3. Gel electrophoresis of digested DNA.

We have successfully cloned A-alpha and A-omega fusions on pKS vector. The DNA will be now sequenced to ensure that our constructs are correct.

Preparation of constructs with OmpA protein fusions

Paweł

1. Isolation of pET15b plasmid.
2. Overnight digest of pET15b plasmid with NdeI and BamHI (Tango 2x buffer), dephosphorylation with CIAP.