Alberta NINT/13 June 2008

From 2008.igem.org

Revision as of 22:49, 11 October 2008 by Wmateri (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

< Back to notebook

< Previous entry | Next entry >


lab work (SD): 

            Using the transformation completed by Wayne (12/06/07) of XL1-B cells with I732018 and the overnight
            cultures, DNA was isolated by QIAprep standard miniprep protocol.
            I732018 was sequenced.
            I732018 was digested with XbaI + PstI.  No evidence of DNA in the gel.  Retry!
            NINT SD p37.jpg

lab work (JD): 

            Quantitated K102005/BamH1 and Nco1
            Ligated K102005/BamH1 and Nco1 with TA0/BamH1 and Nco1 (1/100 dilution) to create plasmid K102007
            Transformed XL1-B cells with K102007 and negative control
            Plated cells and left them for O/N
Retrieved from "http://2008.igem.org/Alberta_NINT/13_June_2008"