Checking if degradation of fusion with OmpA is a result of Top10 proteases' activity (lon, ompt)

Piotr:

Inoculation of OmpA_omega_A_alpha and OmpA_A_alpha with and without inductor (0,5 mm/mL IPTG) in E. coli Rosetta strain.

1. Isolation of plasmids from cultures inocluated on previous day.
2. Control digest of isolated plasmids with BamHI and SacI (we confirmed 4 colonies but A in our constructs turned out to be trunctated and contain only one from two highly similar domains).

Checking for presence of A on the cell membrane

Piotr

Inoculation of omp_A_alfa, omp_Z_alfa and omp_omega_A_alfa (with and without induction).