From 2008.igem.org
Things we did today
Wetlab work
Make Kan50 1000x Concentrate Stock
- Krystle
- Nanopure H2O + Kanamycin
- stored in -20C freezer -> antibiotics box
Make Amp50, Amp50+Kan20 LB Plates
- Krystle & Norman
- made Kanamycin and Ampicillin LB plates according to Drew Endy's Protocol [http://www.openwetware.org/wiki/Endy:Pouring_LB_plates_from_prepped_media here]
- x10 Amp 50uL/mL
- x10 Amp 50ul/mL + Kan 20uL/mL plates
- left plates in hood overnight to dry after pouring (NW), please refrigerate plates in the morning.
Drylab Work
Signal Peptide Research
- Krystle
- found two papers with leader sequences {please post on CiteULike}
- Norman
- blast sequence on Cu chealating protein, found PCC6803 gene and used [http://www.cbs.dtu.dk/services/SignalP/ SignalP 3.0] for signal peptide prediction
Discussion on CO2 Incubator Controller
- Dr. Gautz, Krystle & Norman
- KS will do some fact finding about the parts required
- KS will put together schematic for CO2 regulator design
Discussion
Quote of the Day
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
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