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Team:Hawaii/Notebook/2008-10-14
From 2008.igem.org
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Construction of secretion device (cont.)
- Grace
- Ran RE digests on gel
- Extracted rgt1 and prpgt5 from gel
- Ligated:
- prpgt5 + BBpRL1383a-1
- nir + rgt1 + BBpRL1383a-1
- plac + rgt1 + BBpRL1383a-1
- nrsg6 (from 10/11) + BBpRL1383a-1
- J33207 + pRL1383a
- Transformed E. coli
- Used 5 μl ligation reaction (those containing BBpRL1383a-1) to transform DB3.1 cells
- Used 1 μl ligation reaction (J33207 + pRL1383a) to transform DH5α cells
- Plated 25 μl, 50 μl, 75 μl, and 100 μl of transformed cells on LB+sp2.5+sm2.5 plates
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
