Team:Paris/Modeling/f3bis

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F3env.png
(see the considerations on the use of EnvZ)

We have [EnvZ]produced = {coefenv}expr(pTet) = {coefenv} ƒ1([aTc]i)

and [EnvZ]total = [EnvZ]b + [EnvZ]produced

and [FliA] = {coefFliA}expr(pBad) = {coefFliA} ƒ2([arab]i)

So, if we denote phosphorylated OmpR by OmpR*, we have

F3ompfromenv.jpg

that we can then introduce in the previous expression (see ƒ3) :

F3ompfinalenv.jpg



param signification unit value comments
[expr(pFlhDC)] expression rate of
pFlhDC with RBS E0032
nM.min-1 need for 20 mesures with well choosen values of [aTc]i
γGFP dilution-degradation rate
of GFP(mut3b)
min-1 0.0198
[GFP] GFP concentration at steady-state nM need for 20
(fluorescence) value of the observed fluorescence au need for 20
conversion conversion ratio between
fluorescence and concentration
nM.au-1 (1/79.429)



param signification
corresponding parameters in the equations
unit value comments
(K21eff/{coeffliA}) activation constant of FliA-pFlhDC
K12
nM
n12 complexation order of FliA-pFlhDC
n12
no dimension
β2 production rate of OmpR-pFlhDC with RBS E0032
β2
nM.min-1
(K22/{coefomp}) activation constant of OmpR-pFlhDC
K22
nM
n22 complexation order of OmpR-pFlhDC
n22
no dimension