Team:Mississippi State/15 August 2008

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  • prepare and run gel on plasmid extraction
  • digest plasmid to check for gene
  • there was no insert, and mistake was made in not adding IPTG and PGAL to the plates.
  • Dr Ma: do pcr w/ lip using 2nd primers and taq, load all onto gel, cut out, and purify. Ligate w/ pGEM, transform into e coli, plate, extract