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Team:Valencia/Notebook/September
From 2008.igem.org
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September 2nd
-We carried out the following experiment:
Four strains in LCCs with SP medium.
1 % Galactose induction at the beginning. Initial O.D. 0,7
275 rpm.
-Team meeting: Checking project progress.
September 3rd
-We characterized LCC temperature evolution with water.
September 5th
-We characterized LCC temperature evolution with SP medium and SP medium with bleach (to avoid contamination).
September 8th
- We observed the culture after the experiment under the microcope. There was no contamination but we did observe some elongated microorganisms, same size as S. cerevisiae.
- We discovered that our plaque with the four strains for the following experiment had suffered cross contamination.
September 9th
-We carried out the following experiment:
Four strains in LCCs with SP medium.
1 % Galactose induction at the beginning. Initial O.D. 0,6
270 rpm.
Initial temperature 31 ºC. Too high.
September 11th
-We repeated the same experiment:
Four strains in LCCs with SP medium.
1 % Galactose induction at the beginning. Initial O.D. 0,6
270 rpm.
Initial temperature 31 ºC. Too high again.
September 15th
-We repeated the same experiment:
Four strains in LCCs with SP medium.
1 % Galactose induction at the beginning. Initial O.D. 0,6
270 rpm.
Initial temperature 28 ºC. Perfect!
September 20th
-Trying to reproduce the previous experiment...
Four strains in LCCs with SP medium.
1 % Galactose induction at the beginning. Initial O.D. 0,6
270 rpm.
Initial temperature 28 ºC. But room temperature too high
September 24th
- We repeated the same experiment:
Four strains in LCCs with SP medium.
1 % Galactose induction at the beginning. Initial O.D. 0,6
270 rpm.
Initial temperature 28 ºC.
September 26th
- Team meeting: Checking project progress and inmediate future actions.
September 27th
- We tryied and increased initial O.D.
Four strains in LCCs with SP medium.
1 % Galactose induction at the beginning. Initial O.D. 2
270 rpm.
Initial temperature 28 ºC.
