Checking if degradation of fusion with OmpA is a result of Top10 proteases' activity (lon, ompt)

Piotr:

Inoculation of OmpA_omega_A_alpha and OmpA_A_alpha with and without inductor (0,5 mm/mL IPTG) in E. coli Rosetta strain.

Purification of proteins: Z-alpha and Z-omega

Emilia

100 ml of medium inoculated with overnight culture, then used as inoculum for 1 l culture with presence of ampicillin, kanamycin and IPTG (according to protocol).